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Test Code CTDC Connective Tissue Disease Cascade

Performing Laboratory

Munson Medical Center Laboratories and Mayo Medical Laboratories

Methodology

Enzyme immunoassay (CCP)

Indirect Immunoflourescent Assay (ANA, ds DNA Ab)

 

Reflex testing:

If  ANA is positive, antinuclear antibody (ANA) titer and pattern, extractable nuclear antigen (ENA) screen, and ribosome P antibodies are performed. When appropriate, DNA double-stranded  antibody (Anti-ds DNA) and/or evaluation of antibodies to extractable nuclear antigen performed. All reflex testing performed at additional charge.

 

 

Testing Algorithm

Connective Tissue Disease Cascade

Specimen Requirements

Specimen must arrive within 48 hours of draw.

 

Container/Tube: Gold-top serum gel tube, one full tube

Specimen Volume:  3 mL serum

Transport Temperature: Refrigerate

Collection Instructions:  Centrifuge within 2 hours of draw, then refrigerate.

Reference Values

Cyclic Citrullinated Peptide 3.1 (CCP) Antibody, IgG:

  < 20 U (negative)

20-39 U (weak positive)

40-59 U (moderate positive)

   >59 U (strong positive)

 

Antinuclear Antibody (ANA):

Negative

if positive, reflex testing is performed. See Connective Tissue Disease Cascade for reflex testing algorithm..

 

Days Test(s) Set Up

Mon-Fri: Batched 1-2 times/week

Test Classification and CPT Coding

86201 - CCP antibody

86038 - Antinuclear antibody (ANA)

86039 - Antinuclear antibody titer and pattern

86235x6 - Extractable nuclear antigen screen

83516 - Ribosome P antibodies