Home
HelpTest Code SEIN Semen Analysis Complete (Infertility)
EHR Test Codes
| Test Code | Test Name | |
| Atlas | SEIN | Semen Analysis, Infertility |
| Cerner | Semen Analysis Complete |
Specimen Requirements
Preparation of Patient:
The laboratory recommends refraining from sexual activity including masturbation for 3 - 5 days. Longer or shorter periods of abstinence may result in abnormal results. Follow your physician's instructions if they differ from laboratory recommendation.
The entire ejaculation should be collected in a clean container, by masturbation, and maintained at room and/or body temperature. Do not use lubricants or condom during collection, as they may contain spermicidal agents. Container (not lid) must be labeled with full legal name, date of birth, collection date, and collection time.
Container Type:
Sterile Specimen Cup
Specimen Type:
Semen, total ejaculate
Specimen Volume:
> 1.0 mL minimum < 0.5 mL
Specimen Handling/Transport:
Specimen must be received in laboratory within 1 hour of collection (for assessment of motility and slide making)
Specimen Stability/Storage:
1 hour room temperature
Specimen Rejection:
Specimens collected in a condom
Specimen must be collected in a container that is chemically clean. Soap residues can decrease motility, as can spermicides.
Volume < 0.5 mL
Performing Laboratory
Munson Healthcare Laboratories
Priority, Frequency, & Turnaround
Priority:
Non-Emergent
Frequency:
Monday through Friday 7am - 5pm
Turnaround Time:
0 - 1 day
Methodology
Absorbance spectroscopy and optical density
Reporting
Reference Range:
| Parameter | Range | Unit |
| Semen Volume | 1.4 - 1.7 | mL |
| Semen Viscosity | Normal | |
| Total Sperm (Concentration) | > 15 | M/mL |
| Total Motility | > 40 | % |
| Progressive Motility | > 32 | % |
| Morphology, Semen | > 4 | % |
| pH | > 7.2 |
Interpretive Data:
Semen Volume
A concentrating technique was not performed.
Morphology, Semen
New WHO criteria (5th edition) is in place as of December 14, 2023. The new reference range is >4%.
Critical Decision:
None
Clinical Significance
Volume
In normal individuals the average volume should be between 1.0 - 7.0 mL. Increases of 0.4 mL per day of abstinence can be seen. Decreased volume is associated with infertility but increases are not significant until the volume is greater than 10 mL
Appearance
Any abnormal appearance of the fluid such as blood pigmentation or increased turbidity (due to white blood cells) should be noted
Viscosity
Increased viscosity that affects motility is significant because there may be poor penetration of the cervical mucus by spermatozoa
Motility
Sperm motility of greater than 60% is associated with fertility
Count
Although the normal range is >20 million/mL, counts of between 10 and 20 million are considered borderline. 40-50% of infertility problems are said to be due to dysfunction of the male reproductive system
pH
pH of normal semen is slightly alkaline. An abnormally high ratio of prostatic fluid to seminal fluid will produce a more acid pH
CPT Code(s)
89310