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Test Code NAIFA Antinuclear Antibodies, HEp-2 Substrate, IgG, Serum

Useful For

Evaluating patients suspected of having systemic rheumatic disease

Method Name

Indirect Immunofluorescence

Reporting Name

Antinuclear Ab, HEp-2 Substrate, S

Specimen Type


Specimen Required

Container/Tube: Serum gel or red top

Specimen Volume: 0.5 mL

Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 21 days
  Frozen  28 days

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK

Reference Values

<1:80 (Negative)

Day(s) and Time(s) Performed

Monday through Saturday; 11 a.m.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
NAIFA Antinuclear Ab, HEp-2 Substrate, S 59069-5


Result ID Test Result Name Result LOINC Value
ANAH Antinuclear Ab, HEp-2 Substrate, S 59069-5
1TANA ANA Titer: 33253-6
1PANA ANA Pattern: 49311-4
2TANA ANA Titer 2: 33253-6
2PANA ANA Pattern 2: 49311-4
CYTQL Cytoplasmic Pattern: 55171-3
LCOM Lab Comment: 77202-0


If not ordering electronically, complete, print, and send a General Request (T239) with the specimen.