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HelpTest Code MSAES Myositis Specific Antibody Evaluation, Serum
Ordering Guidance
This test is appropriate for patients presenting with proximal muscle weakness, elevated muscle enzymes (eg, creatine kinase), and/or suggestive cutaneous features (eg, heliotrope rash, Gottron’s papules) consistent with myositis and related disorders. This test can assist in classifying IIM subtypes (eg, dermatomyositis, anti-synthetase syndrome, immune-mediated necrotizing myopathy, or inclusion body myositis. It may be useful in distinguishing idiopathic inflammatory myopathy from overlapping connective tissue diseases (CTDs). For patients with suspicion of overlap syndromes with CTDs additional myositis-associated antibody testing may be warranted beyond this panel.
Additional Testing Requirements
In patients with atypical or non-classical presentations testing for some myositis associated antibodies may be considered including anti-U1-snRNP, PM/Scl, Ro52 or Ro60.
Necessary Information
Provide the following information:
-Relevant clinical information
-Ordering provider name, phone number, mailing address, and e-mail address
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Red top
Acceptable: Serum gel
Submission Container/Tube: Plastic vial
Specimen Volume: 2.5 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Forms
If not ordering electronically, complete, print, and send a Neurology Specialty Testing Client Test Request (T732) with the specimen.
Useful For
Accurately diagnosing, classifying, and managing idiopathic inflammatory myopathies (IIM) by identifying subtype-specific biomarkers that guide prognosis and treatment
Enabling early detection of IIM, particularly in atypical or severe cases, and reducing diagnostic uncertainty for personalized care
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| EJS | EJ Ab, S | No | Yes |
| HMGCR | HMG-CoA Reductase Ab, S | Yes | Yes |
| JO1 | Jo 1 Ab, IgG, S | Yes | Yes |
| MDA5S | MDA5 Ab, S | No | Yes |
| MI2S | Mi2 Ab, S | No | Yes |
| MYSI | Myositis Specific Ab Interp, S | No | Yes |
| NXP2S | NXP2 Ab, S | No | Yes |
| OJS | OJ Ab, S | No | Yes |
| PL12S | PL12 Ab, S | No | Yes |
| PL7S | PL7 Ab, S | No | Yes |
| SAE1S | SAE1 Ab, S | No | Yes |
| SRPIS | SRP IFA Screen, S | No | Yes |
| TIFGS | TIF1G Ab, S | No | Yes |
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| SRPBS | SRP Immunoblot, S | No | No |
| SRPTS | SRP IFA Titer, S | No | No |
Testing Algorithm
If the indirect immunofluorescence assay (IFA) pattern suggests signal recognition particle (SRP) antibody, then the SRP IFA titer and SRP54 immunoblot will be performed at an additional charge.
Method Name
EJS, MDA5S, MI2S, NXP2S, OJS, PL12S, PL7S, SAE1S, TIFGS: Bead-Based Multi-Analyte Immunoassay
JO1: Multiplex Flow Immunoassay (MFI)
SRPIS, SRPTS: Indirect Immunofluorescence Assay (IFA)
HMGCR: Chemiluminescence Immunoassay (CIA)
SRPBS: Immunoblot (IB)
MYSI: Technical Interpretation
Reporting Name
Myositis Specific Ab Eval, SSpecimen Type
SerumSpecimen Minimum Volume
1.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerated (preferred) | 21 days |
| Frozen | 21 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | Reject |
| Heat-Treated | Reject |
Reference Values
|
Test ID |
Reporting Name |
Methodology* |
Reference Value |
|
MYSI |
Myositis Specific Ab Interp, S |
Technical interpretation |
Interpretive report |
|
EJS |
EJ Ab, S |
PMAT |
Negative |
|
HMGCR |
HMG-CoA Reductase Ab, S |
CIA |
<20.0 |
|
JO1 |
Jo 1 Ab, IgG, S |
MFI |
<1.0 U |
|
MDA5S |
MDA5 Ab, S |
PMAT |
Negative |
|
MI2S |
Mi2 Ab, S |
PMAT |
Negative |
|
NXP2S |
NXP2 Ab, S |
PMAT |
Negative |
|
OJS |
OJ Ab, S |
PMAT |
Negative |
|
PL12S |
PL12 Ab, S |
PMAT |
Negative |
|
PL7S |
PL7 Ab, S |
PMAT |
Negative |
|
SAE1S |
SAE1 Ab, S |
PMAT |
Negative |
|
SRPIS |
SRP IFA Screen, S |
IFA |
Negative |
|
TIFGS |
TIF1G Ab, S |
PMAT |
Negative |
*Methodology abbreviations:
Bead-based multi-analyte immunoassay (PMAT)
Multiplex flow immunoassay (MFI)
Indirect immunofluorescence assay (IFA)
Chemiluminescence immunoassay (CIA)
Immunoblot (IB)
Day(s) Performed
Varies
Report Available
3 to 7 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
86255 x10
82397
86235
84182-SRPBS (if appropriate)
86256-SRPTS (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| MSAES | Myositis Specific Ab Eval, S | In Process |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 607414 | HMG-CoA Reductase Ab, S | 93493-5 |
| 622115 | Myositis Specific Ab Interp, S | 69048-7 |
| 622112 | TIF1G Ab, S | In Process |
| 622114 | SAE1 Ab, S | In Process |
| 622108 | PL7 Ab, S | 33772-5 |
| 622109 | PL12 Ab, S | 33771-7 |
| 622074 | OJ Ab, S | 45152-6 |
| 622110 | NXP2 Ab, S | In Process |
| 622113 | Mi2 Ab, S | 18485-3 |
| 622111 | MDA5 Ab, S | In Process |
| 621604 | EJ Ab, S | 45149-2 |
| JO1 | Jo 1 Ab, IgG, S | 33571-1 |
| 603540 | SRP IFA Screen, S | 97562-3 |