Sign in →

Test Code MDS2 Movement Disorder, Autoimmune Evaluation, Serum


Necessary Information


Provide the following information:

-Relevant clinical information

-Ordering provider name, phone number, mailing address, and e-mail address



Specimen Required


Patient Preparation:

1. For optimal antibody detection, specimen collection is recommended prior to initiation of immunosuppressant medication.

2. This test should not be requested in patients who have recently received radioisotopes, therapeutically or diagnostically, because of potential assay interference. The specific waiting period before specimen collection will depend on the isotope administered, the dose given, and the clearance rate in the individual patient. Specimens will be screened for radioactivity prior to analysis. Radioactive specimens received in the laboratory will be held 1 week and assayed if sufficiently decayed, or canceled if radioactivity remains.

Container/Tube:

Preferred: Red top

Acceptable: Serum gel

Specimen Volume: 4 mL


Useful For

Evaluating patients with suspected paraneoplastic or other autoimmune movement disorders including patients with ataxia, chorea, dyskinesias, myoclonus, parkinsonism, and stiff-person spectrum in serum specimens

Profile Information

Test ID Reporting Name Available Separately Always Performed
MDSI Movement Disorder Interp, S No Yes
GANG AChR Ganglionic Neuronal Ab, S No Yes
AMPHS Amphiphysin Ab, S No Yes
AGN1S Anti-Glial Nuclear Ab, Type 1 No Yes
ANN1S Anti-Neuronal Nuclear Ab, Type 1 No Yes
ANN2S Anti-Neuronal Nuclear Ab, Type 2 No Yes
ANN3S Anti-Neuronal Nuclear Ab, Type 3 No Yes
CS2CS CASPR2-IgG CBA, S No Yes
CRMS CRMP-5-IgG, S No Yes
CRMWS CRMP-5-IgG Western Blot, S Yes Yes
DPPIS DPPX Ab IFA, S No Yes
GD65S GAD65 Ab Assay, S Yes Yes
LG1CS LGI1-IgG CBA, S No Yes
GL1IS mGluR1 Ab IFA, S No Yes
NMDCS NMDA-R Ab CBA, S No Yes
CCN N-Type Calcium Channel Ab No Yes
CCPQ P/Q-Type Calcium Channel Ab No Yes
PCABP Purkinje Cell Cytoplasmic Ab Type 1 Yes Yes
PCAB2 Purkinje Cell Cytoplasmic Ab Type 2 Yes Yes
PCATR Purkinje Cell Cytoplasmic Ab Type Tr No Yes

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
ABLOT Amphiphysin Western Blot, S No No
AMPIS AMPA-R Ab IF Titer Assay, S No No
AMPCS AMPA-R Ab CBA, S No No
DPPCS DPPX Ab CBA, S No No
DPPTS DPPX Ab IFA Titer, S No No
GABCS GABA-B-R Ab CBA, S No No
GABIS GABA-B-R Ab IF Titer Assay, S No No
GL1CS mGluR1 Ab CBA, S No No
GL1TS mGluR1 Ab IFA Titer, S No No
NMDIS NMDA-R Ab IF Titer Assay, S No No
WBN Paraneoplastic Autoantibody WBlot,S No No

Testing Algorithm

If indirect immunofluorescence assay (IFA) pattern suggests ANN1S, ANN2S, ANN3S, PCABP, PCAB2, PCATR, or AGN1S, then paraneoplastic autoantibody Western blot is performed at an additional charge.

 

If IFA pattern suggests amphiphysin antibody, then amphiphysin Western blot is performed at an additional charge.

 

If IFA pattern suggests AMPA-R antibody, then AMPA-R cell-binding assay (CBA) and AMPA-R titer are performed at an additional charge.

 

If IFA pattern suggests DPPX antibody, then DPPX CBA and DPPX titer are performed at an additional charge.

 

If IFA pattern suggests GABA-B-R antibody, then GABA-B-R CBA and GABA-B-R titer are performed at an additional charge.

 

If IFA pattern suggests mGluR1 antibody, then mGluR1 CBA and mGluR1 titer are performed at an additional charge.

 

If IFA pattern suggests NMDA-R antibody and NMDA-R CBA is positive, then NMDA-R titer is performed at an additional charge.

 

See Movement Disorder Autoimmune Evaluation Algorithm-Serum in Special Instructions.

Method Name

ANN1S, ANN2S, ANN3S, PCABP, PCAB2, PCATR, AMPHS, CRMS, AGN1S, DPPIS, DPPTS, GL1IS, GL1TS, AMPIS, GABIS, NMDIS: Indirect Immunofluorescence Assay (IFA)

AMPCS, GABCS, NMDCS, LG1CS, CS2CS, DPPCS, GL1CS: Cell Binding Assay (CBA) 

ABLOT, CRMWS, WBN: Western Blot (WB)

 

CCN, CCPQ, GANG ,GD65S: Immunoprecipitation Assay (IPA)

Reporting Name

Movement Autoimmune Eval, S

Specimen Type

Serum

Specimen Minimum Volume

3 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 28 days
  Frozen  28 days
  Ambient  72 hours

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject

Reference Values

Test ID

Reporting Name

Methodology

Reference Value

GANG

AChR Ganglionic Neuronal Ab, S

Radioimmunoassay (RIA)

≤0.02 nmol/L

AMPHS

Amphiphysin Ab, S

Immunofluorescence assay (IFA)

<1:240

AGN1S

Anti-Glial Nuclear Ab, Type 1

IFA

<1:240

ANN1S

Anti-Neuronal Nuclear Ab, Type 1

IFA

<1:240

ANN2S

Anti-Neuronal Nuclear Ab, Type 2

IFA

<1:240

ANN3S

Anti-Neuronal Nuclear Ab, Type 3

IFA

<1:240

CS2CS

CASPR2-IgG CBA, S

Cell-binding assay (CBA)

Negative

CRMS

CRMP-5-IgG, S

IFA

<1:240

CRMWS

CRMP-5-IgG Western Blot, S

Western blot (WB)

Negative

DPPIS

DPPX Ab IFA, S

IFA

Negative

GD65S

GAD65 Ab Assay, S

Immunoprecipitation assay (IPA)

≤0.02 nmol/L

Reference values apply to all ages. 

LG1CS

LGI1-IgG CBA, S

CBA

Negative

GL1IS

mGluR1 Ab IFA, S

IFA

Negative

NMDCS

NMDA-R Ab CBA, S

CBA

Negative

CCN

N-Type Calcium Channel Ab

RIA

≤ 0.03 nmol/L 

CCPQ

P/Q-Type Calcium Channel Ab

RIA

≤0.02 nmol/L

PCABP

Purkinje Cell Cytoplasmic Ab Type 1

IFA

<1:240

PCAB2

Purkinje Cell Cytoplasmic Ab Type 2

IFA

<1:240

PCATR

Purkinje Cell Cytoplasmic Ab Type Tr

IFA

<1:240

Reflex Information:

Test ID

Reporting Name

Methodology

Reference Value

ABLOT

Amphiphysin Western Blot, S

WB

Negative

AMPIS

AMPA-R Ab IF Titer Assay, S

IFA

<1:120

AMPCS

AMPA-R Ab CBA, S

CBA

Negative

DPPCS

DPPX Ab CBA, S

CBA

Negative

DPPTS

DPPX Ab IFA Titer, S

IFA

<1:240

GABCS

GABA-B-R Ab CBA, S

CBA

Negative

GABIS

GABA-B-R Ab IF Titer Assay, S

IFA

<1:120

GL1CS

mGluR1 Ab CBA, S

CBA

Negative

GL1TS

mGluR1 Ab IFA Titer, S

IFA

<1:240

NMDIS

NMDA-R Ab IF Titer Assay, S

IFA

<1:120

WBN

Paraneoplastic Autoantibody WBlot,S

WB

Negative

 

Neuron-restricted patterns of IgG staining that do not fulfill criteria for ANNA-1, ANNA-2, CRMP-5-IgG, PCA-1, PCA-2, or PCA-Tr may be reported as "unclassified anti-neuronal IgG." Complex patterns that include nonneuronal elements may be reported as "uninterpretable."

Day(s) and Time(s) Performed

ANN1S, ANN2S, ANN3S, PCABP, PCAB2, PCATR, AMPHS, CRMS, AGN1S, DPPIS, DPPTS, GL1IS, GL1TS, AMPIS, GABIS, NMDIS:

Monday through Friday; 5 a.m., 7 a.m., 5 p.m.

Saturday, Sunday; 6 a.m.

 

GANG, CCN, CCPQ:

Monday through Friday; 6 a.m., 8 a.m., 6 p.m.

Saturday, Sunday; 7 a.m.

 

CS2CS, LG1CS, NMDCS, AMPCS, GABCS:

Monday through Thursday; 10 p.m.

Sunday; 3 p.m.

 

CRMWS, ABLOT, WBN:

Monday, Wednesday, Friday; 8 a.m.

 

GD65S:

Monday-Friday; 5:00 a.m., 2:00 p.m.

Saturday and Sunday; 7:00 a.m.

 

DPPCS, GL1CS:

Wednesday; 6 p.m.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

83519 x3

86255 x14

84182 x1

86341 x1

86255 x4 (if appropriate)

86256 x5 (if appropriate)

84182 x2 (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MDS2 Movement Autoimmune Eval, S In Process

 

Result ID Test Result Name Result LOINC Value
61516 NMDA-R Ab CBA, S 93503-1
64279 LGI1-IgG CBA, S In Process
64281 CASPR2-IgG CBA, S In Process
64930 DPPX Ab IFA, S 82976-2
64928 mGluR1 Ab IFA, S In Process
601998 Movement Disorder Interp, S 69048-7
89080 AGNA-1, S 53709-2
81722 Amphiphysin Ab, S 33927-5
80150 ANNA-1, S 13997-2
80776 ANNA-2, S 43188-2
83137 ANNA-3, S 33924-2
81184 N-Type Calcium Channel Ab 33979-6
81185 P/Q-Type Calcium Channel Ab 33980-4
83077 CRMP-5-IgG, S 35386-2
83107 CRMP-5-IgG Western Blot, S 47401-5
84321 AChR Ganglionic Neuronal Ab, S 42233-7
81596 GAD65 Ab Assay, S 30347-9
83138 PCA-2, S 33925-9
9477 PCA-1, S 53717-5
83076 PCA-Tr, S 56550-7
36349 Reflex Added 77202-0

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.