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Test Code LPMGF Lymphocyte Proliferation to Mitogens, Blood

Reporting Name

Lymphocyte Proliferation, Mitogens

Useful For

Assessing T-cell function in patients on immunosuppressive therapy, including solid-organ transplant patients

 

Evaluating patients suspected of having impairment in cellular immunity

 

Evaluation of T-cell function in patients with primary immunodeficiencies, either cellular (DiGeorge syndrome, T-negative severe combined immunodeficiency: SCID, etc) or combined T- and B-cell immunodeficiencies (T- and B-negative SCID, Wiskott Aldrich syndrome, ataxia telangiectasia, common variable immunodeficiency, among others) where T-cell function may be impaired

 

Evaluation of T-cell function in patients with secondary immunodeficiency, either disease related or iatrogenic

 

Evaluation of recovery of T-cell function and competence following bone marrow transplantation or hematopoietic stem cell transplantation

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

WB Sodium Heparin


Advisory Information


 



Shipping Instructions


Specimens are required to be received in the laboratory weekdays and by 4 p.m. on Friday. Draw and package specimen as close to shipping time as possible. Ship specimen overnight in an Ambient Shipping Box-Critical Specimens Only (T668 following the instructions in the box).

 

It is recommended that specimens arrive within 24 hours of draw.

 

Specimens arriving on the weekend may be canceled.



Necessary Information


1. Date and time of draw and ordering physician name and phone number are required.

2. Specify "Mitogen" to differentiate from "Antigen" testing. This information is required.



Specimen Required


For serial monitoring, we recommend that specimen draws be performed at the same time of day.

 

Supplies: Ambient Shipping Box-Critical Specimens Only (T668)

Container/Tube: Green top (sodium heparin)

Specimen Volume:

<3 months: 1 mL

3 months-5 years: 2 mL

6-18 years: 3 mL

>18 years: 10 mL

Collection Instructions: Send specimen is original tube. Do not aliquot.

 

Blood Volume Recommendations Based on Absolute Lymphocyte Count (ALC)

 

Mitogen Only

ALC

Blood Volume for Minimum PHA Only

Blood Volume for Minimum PHA and PWM

Blood Volume for Full Assay

<0.5

>6.5 cc

>8.5 cc

>22 cc

0.5-1.0

6.5 cc

8.5 cc

22 cc

1.1-1.5

3.0 cc

4.0 cc

10 cc

1.6-2.0

2.0 cc

2.5 cc

7 cc

2.1-3.0

1.5 cc

2.0 cc

6 cc

3.1-4.0

1.0 cc

1.5 cc

4 cc

4.1-5.0

0.8 cc

1.0 cc

3 cc

>5.0

0.5 cc

0.8 cc

2 cc

 

Mitogen and Antigen

ALC

Blood Volume for Minimum of Each Assay

Blood Volume for Full Assay

<0.5

>28 cc

>60 cc

0.5-1.0

28 cc

60 cc

1.1-1.5

12 cc

30 cc

1.6-2.0

8.5 cc

20 cc

2.1-3.0

6.5 cc

15 cc

3.1-4.0

4.5 cc

10 cc

4.1-5.0

3.5 cc

8 cc

>5.0

2.5 cc

6 cc


Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
WB Sodium Heparin Ambient 48 hours GREEN TOP/HEP

Reference Values

Viability of lymphocytes at day 0: ≥75.0%

Maximum proliferation of phytohemagglutinin as % CD45: ≥49.9%

Maximum proliferation of phytohemagglutinin as % CD3: ≥58.5%

Maximum proliferation of pokeweed mitogen as % CD45: ≥4.5%

Maximum proliferation of pokeweed mitogen as % CD3: ≥3.5%

Maximum proliferation of pokeweed mitogen as % CD19: ≥3.9%

Day(s) and Time(s) Performed

Monday through Friday

Do not send specimen after Thursday.

Test Classification

This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

86353

86353 (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
LPMGF Lymphocyte Proliferation, Mitogens 69018-0

 

Result ID Test Result Name Result LOINC Value
32317 Interpretation 69052-9
32318 Viab of Lymphs at Day 0 33193-4
32321 Max Prolif of PWM as % CD45 69019-8
32322 Max Prolif of PWM as % CD3 69020-6
32323 Max Prolif of PWM as % CD19 69037-0
32319 Max Prolif of PHA as % CD45 69038-8
32320 Max Prolif of PHA as % CD3 57741-1
32324 Mitogen Comment 48767-8

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject

Method Name

Flow Cytometry

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
MGSTM Additional Flow Stimulant, LPMGF No, (Bill Only) No

Testing Algorithm

If insufficient peripheral blood mononuclear cells (PBMCs) are isolated from the patient's sample due to low WBC counts or specimen volume received, selected dilutions or stimulants may not be tested at the discretion of the laboratory to ensure the most reliable results. Testing with 1 stimulant will always be performed. When adequate specimen is available for both stimulants to be tested, an additional test ID will be reflexed and billed separately.