Test Code HSVPS HSV1 & HSV2, IgG, Serum

Methodology

Chemiluminescent immunoassay (CLIA)

Preparation of Patient:

None

Specimen Type:

Serum

Container Type:

Gold Top SST Gel & Clot Activator Tube

LIS Container Code = GL

Specimen Volume:

1.0 mL of Serum minimum 400uL of Serum

Specimen Handling/Transport:

Separate serum from cells within 2 hours of collection

For shipping, specimens should be maintained at 2-8°C.

Specimen Stability/Storage:

2 hours ambient

5 days 2-8°C

Reference Ranges: Negative

Critical decision level: None

Result Interpretation: The LIAISON® Analyzer automatically calculates HSV-1 IgG levels expressed as Index value and grades the results.

Index Value	Results	Interpretation
< 0.90 Index	Negative	No detectable antibodies to HSV-1 were found. A negative result generally indicates that the patient has not been infected, but does not always rule out acute HSV infection. If clinical exposure to HSV is suspected despite a negative finding, a second sample should be collected and tested no less than 4 – 6 weeks later.
≥ 0.90 Index and < 1.10 Index	Equivocal	Equivocal samples should be retested in duplicate by the LIAISON HSV-1 assay in order to confirm the initial result or tested by a lab experienced in confirmatory methods such as Western Blot. Samples which are positive (≥ 1.10) at the second test should be considered positive. Samples which are negative (<0.90) at the second test should be considered negative. A second sample shou be collected and tested no less than 4 – 6 weeks later when the result is repeatedly equivocal.
≥ 1.10 Index	Positive	Indicates the presence of detectable IgG antibody to HSV-1

Reference Ranges: Negative

Critical decision level: None

Result Interpretation: The LIAISON® Analyzer automatically calculates HSV-2 IgG levels expressed as Index value and grades the results.

Index Value	Results	Interpretation
< 0.90 Index	Negative	No detectable antibodies to HSV-2 were found. A negative result generally indicates that the patient has not been infected, but does not always rule out acute HSV infection. If clinical exposure to HSV is suspected despite a negative finding, a second sample should be collected and tested no less than 4 – 6 weeks later.
≥ 0.90 Index and < 1.10 Index	Equivocal	Equivocal samples should be retested in duplicate by the LIAISON HSV-2 assay in order to confirm the initial result or tested by a lab experienced in confirmatory methods such as Western Blot. Samples which are positive (≥ 1.10) at the second test should be considered positive. Samples which are negative (<0.90) at the second test should be considered negative. A second sample shou be collected and tested no less than 4 – 6 weeks later when the result is repeatedly equivocal.
≥ 1.10 Index	Positive	Indicates the presence of detectable IgG antibody to HSV-2

86695

86696

Non-Emergent

Tuesday & Friday