Test Code GPTF, GPT1, GPT2, GPT3 Glucose Tolerance Gestational 3-Hour
Methodology
Glucose Oxidase
Performing Laboratory
Munson Healthcare Laboratories
Specimen Requirements
Patient Preparation
1. A fasting (8 hour) specimen is drawn.
2. Patient is given 100 g GlucolaTM.
3. Blood is to be drawn every hour for the next 3 hours.
4. Instruct patient as follows:
A. No food should be taken during the test period
B. No drinks, only sips of water, throughout test.
C. No smoking during the tes period
D. Limited exercise
E. Inform laboratory or nursing staff when symptoms of nausea, vomiting, dizziness, dyspnea, or fainting occur.
Specimen Collection
Specimen must arrive within 72 hours of draw.
Submit only 1 of the following specimens:
Plasma
Container/Tube: Green-top (heparin) gel tube or grey-top (potassium oxalate/sodium fluoride) tube
Specimen Volume: 1 mL of heparinized plasma or potassium oxalate/sodium fluoride plasma
Transport Temperature: Refrigerate
Collection Instructions: Fasting (8 hour). Centrifuge within 1 hour of draw. For grey-top (potassium oxalate/sodium fluoride tube, do not centrifuge. Forward promptly.
Note: 1. Indicate plasma.
2. Label specimen appropriately (plasma).
Serum
Container/Tube: Gold-top serum gel tube
Specimen Volume: 0.5 mL of serum
Transport Temperature: Refrigerate
Collection Instructions: Fasting (8 hour). Centrifuge within 1 hour of draw. Forward promptly.
Note: 1. Indicate serum.
2. Label specimen appropriately (serum).
Reference Values
Fasting: <105 mg/dL
1-Hour: <190 mg/dL
2-Hour: <165 mg/dL
3-Hour: <145 mg/dL
Critical values (automatic call-back):
Pediatric patients up to 21 years old ≤ 35 mg/dL, ≥ 200 mg/dL
Patients 21 years and older ≤ 50mg/dL, ≥500 mg/dL
Day(s) Test Set Up
Monday through Sunday
Test Classification and CPT Coding
82951-Tolerance test (GTT), 3 specimens (includes glucose)
82952-GTT, each additional beyond 3 specimens