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Test Code AFPA Alpha-Fetoprotein, Amniotic Fluid

Reporting Name

Alpha Fetoprotein, AF

Useful For

Screening for open neural tube defects or other fetal abnormalities


Follow-up testing for patients with elevated serum alpha-fetoprotein results or in conjunction with cytogenetic testing

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
ACHE_ Acetylcholinesterase, AF Yes No

Testing Algorithm

If alpha-fetoprotein (AFP) is positive, then acetylcholinesterase (AChE) will be performed at an additional charge.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Amniotic Fld

Specimen Required

Container/Tube: Amniotic fluid container

Specimen Volume: 1 mL

Collection Instructions: Do not centrifuge.

Additional Information

1. The following information is required:

a. Date ultrasound performed

b. Estimated due date by ultrasound

c. Collection date

d. Gestational age must be between 13 and 24 weeks; 16 to 18 weeks preferred.

2. If chromosome studies are also requested, see AF / Chromosome Analysis, Amniotic Fluid. The specimen for AFP-AF testing, when requested with chromosome analysis, cannot be frozen.

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Amniotic Fld Refrigerated 7 days

Reference Values

≤2.0 multiples of median (MoM)

Day(s) and Time(s) Performed

Monday through Friday; 5 a.m.-5 p.m., Saturday; 6 a.m.-1 p.m.

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information


82013-Acetylcholinesterase (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
AFPA Alpha Fetoprotein, AF 58735-2


Result ID Test Result Name Result LOINC Value
6739 Collection Date 58734-5
DAT15 EDD by US Scan 11781-2
6740 Last Menstrual Period (LMP) 8665-2
24233 EDD by LMP 11779-6
24239 GA at Collection by Scan 11888-5
24240 GA at Collection by Dates 11885-1
24234 GA Used 21299-3
9950 Alpha Fetoprotein, AF 29595-6
24241 Results 43798-8
24235 Interpretation 59462-2
24236 Additional Comments 48767-8
24237 Follow up 80615-8
24238 General Test Info 48767-8

Reject Due To

No specimen should be rejected.

Method Name

AFPA: Immunoenzymatic Assay
ACHE_: Polyacrylamide Electrophoresis