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Test Code MTRTI MatePair, Targeted Rearrangements, Oncology

Useful For

Second-tier testing in oncologic specimens when previous cytogenetic testing has detected an acquired chromosome abnormality of unknown significance

 

Determining the size, precise breakpoints, gene content, and any unappreciated complexity of abnormalities detected by other methods such as conventional chromosome and FISH studies

 

Providing important diagnostic, prognostic, and therapeutic information critical to proper patient management

Method Name

Mate-Pair Whole Genome Sequencing

Reporting Name

MatePair, Targeted, Oncology

Specimen Type

Tissue


Shipping Instructions


Advise Express Mail or equivalent if not on courier service.



Necessary Information


1. Previous cytogenetic or FISH testing is required in order to perform this test. If previous testing was performed at another institution, submit a copy of the results or the Mate Pair Hematology/Oncology Patient Information (see Special Instructions). Testing will be canceled if sufficient information regarding the patient's known chromosome abnormality is not made available.

2. A reason for referral must be provided for testing to be performed.

3. A pathology report should accompany the specimen. If this information is not available at the time of order, submit as soon as possible for appropriateness of testing and to aid in interpretation of results.



Specimen Required


Submit only 1 of the following specimens:

 

Supplies: Hank's Solution (T132)

Specimen Type: Tumor biopsy

Container/Tube: Sterile container with sterile Hank's balanced salt solution (T132), Ringer's solution, or normal saline

Specimen Volume: 0.5-3 cm(3) or larger

 

Supplies: Hank's Solution (T132)

Specimen Type: Lymph node

Container/Tube: Sterile container with sterile Hank's balanced salt solution (T132), Ringer's solution, or normal saline.

Specimen Volume: 1 cm(3)

 

Supplies: Hank's Solution (T132)

Specimen Type: Skin biopsy

Container/Tube: Sterile container with sterile Hank's balanced salt solution (T132), Ringer's solution, or normal saline.

Specimen Volume: 4-mm diameter

Collection Instructions:

1. Wash biopsy site with an antiseptic soap.

2. Thoroughly rinse area with sterile water.

3. Do not use alcohol or iodine preparations.

4. A local anesthetic may be used.

5. Biopsy specimens are best taken by punch biopsy to include full thickness of dermis.

 

Specimen Type: Snap Frozen tissue

Container/Tube: Polycon flat

Specimen Volume: 3 cm(3)/Lymph Node: 1 cm(3)/Skin Biopsy: 4 mm diameter

 

Specimen Type: Cultured tumor cells

Container/Tube: T-25 flask filled with transport media

Specimen Volume: T-25 flask

 

Specimen Type: Cryo-frozen cultured tumor cells

Container/Tube: Sterile container in media with DMSO or other safe cryopreservation solution

Specimen Volume: 5 million cells

 

Specimen Type: Cryo-Frozen tissue

Container/Tube: Polycon flat

Specimen Volume: 3 cm(3)/Lymph Node: 1 cm(3)/Skin Biopsy: 4-mm diameter


Specimen Minimum Volume

Tumor Biopsy: 3 cm(3)
Lymph Node: 1 cm(3)
Skin Biopsy: 4-mm diameter
Cultured tumor cells: T-25 flask
Cryo-frozen cultured tumor cells: 5 million cells

Specimen Stability Information

Specimen Type Temperature Time Special Container
Tissue Varies

Reject Due To

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Reference Values

An interpretive report will be provided.

Day(s) and Time(s) Performed

Samples processed Monday through Friday. Results reported Monday through Friday, 8 a.m.-5 p.m.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

0013U

 

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MTRTI MatePair, Targeted, Oncology In Process

 

Result ID Test Result Name Result LOINC Value
48023 Result Summary 50397-9
48326 Result 48001-2
48024 Nomenclature 62356-1
48025 Interpretation 69965-2
CG988 Reason for Referral 42349-1
CG986 Specimen 31208-2
48028 Source 31208-2
48029 Method 49549-9
48030 Additional Information 48767-8
48032 Released By 18771-6

Forms

1. New York Clients-Informed consent is required. Document on the request form or electronic order that a copy is on file. The following documents are available in Special Instructions:

-Informed Consent for Genetic Testing (T576)

-Informed Consent for Genetic Testing-Spanish (T826)

2. Mate Pair Hematology/Oncology Patient Information in Special Instructions

3. If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

-Oncology Test Request (T729)

-Hematopathology/Cytogenetics Test Request (T726)